Ureaplasma parvum/urealyticum RT-PCR (CE) | B19-100FRT

Ureaplasma parvum/urealyticum RT-PCR | B19-100FRT is available for delivery

Description:

General information: Real Time PCR test for detection of Ureaplasma parvum/urealyticum

Target Disease Type: Sexually trasmitted diseases

Specific Application: Ureaplasma species and Mycoplasma

Storage and Shipping : stock

Ureaplasma parvum/urealyticum RT-PCR (CE) B19-100FRT DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gonorrhea, herpes, HIV, HPV, syphilis, gardnerella and trichomoniasis.

Bacterial vaginosis (BV) is a lower genital tract infection characterized by the presence of thin, white, homogeneous, fishy-smelling vaginal discharge. This discharge is present in the absence of signs of vaginal irritation, such as pain, itching, burning, soreness, and dyspareunia. As such, in reference to the lack of demonstrable inflammatory response, the term 'vaginosis' was adopted instead of vaginitis. Bacterial vaginosis is characterized by a disruption of the normal vaginal equilibrium.The lactobacilli population decreases, which leads to an increase in vaginal pH (as high as 7.0) and overgrowth of and replacement by vaginosis-associated anaerobic microorganisms. Up to 90% of women with bacterial vaginosis harbor Gardnerella vaginalis.

Other associated microbial populations identified include Prevotella bivia, Mobiluncus species, Gram-positive cocci, Bacteroides, Mycoplasma hominis, Ureaplasma, Megasphaera, and Leptotrichia. Atopobium vaginae, Streptococcus.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD and BV diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

Kit Ur.parvum/urealyticum Real-TM is a multiplex Real Time PCR test for the qualitative detection of Ureaplasma parvum and Ureaplasma urealyticum in the urogenital swabs, urine, prostatic liquid and other biological materials.

PRINCIPLE OF ASSAY

kit Ur. parvum/urealyticum Real-TM is based on two major processes: isolation of DNA from specimens and Real Time amplification. Ureaplasma parvum/urealyticum DNA is extracted from the specimens, amplified using Real-Time amplification. and detected using fluorescent reporter dye probes specific for Ureaplasma DNA and Internal Control. Internal Control (IC) serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition. Ureaplasma parvum is detected on the FAM (Green) channel, Ureaplasma urealyticum on the JOE (Yellow)/Cy3/HEX channel and IC DNA on the ROX (Orange)/Texas Red channel.