Urease Inhibitor Screening Kit (Colorimetric) - K2079

K2079 | Urease Inhibitor Screening Kit (Colorimetric) DataSheet

Urease (Urea Aminohydrolase, E.C.3.5.1.5) is a nickel-dependent metalloenzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide. Urease is produced by plants, bacteria, fungi and some invertebrates. Bacterial urease is of special importance since it is a major virulence factor caused by bacteria during infections. It is essential in the colonization of host organism and in the maintenance of bacterial cells in tissues. Ureases hydrolyze urea secreted in the blood and saliva to release ammonia, which is the major factor contributing to infections such as urinary tract and gastroduodenal infections, urinary catheter encrustation, stomach cancer, kidney stone formation, pneumonia etc. Thus, controlling the ureolytic activity of microorganism is a promising target to treat diseases caused by bacterial ureases. ’s Urease Inhibitor Screening Kit provides a quick, easy and sensitive assay to screen and characterize potential urease inhibitors. In this assay, urease of Canavalia ensiformis is used to screen urease inhibitors. The production of ammonia is measured at 670 nm using a modified Berthelot method and is applied to determine the inhibition efficiency of the screened compounds. A control Urease Inhibitor is also included in the kit.

Alternate Name : Urease Inhibitor Screening Urease Inhibitor Screening (Colorimetric) Urease Inhibitor Screening Kit

Tag Line : For screening or characterizing urease inhibitors

Summary : ’s Urease Inhibitor Screening Kit provides a quick, easy and sensitive assay to screen and characterize potential urease inhibitors. In this assay, urease of Canavalia ensiformis is used to screen urease inhibitors. The production of ammonia is measured at 670 nm using a modified Berthelot method and is applied to determine the inhibition efficiency of the screened compounds. A control Urease Inhibitor is also included in the kit.

Detection Method : Absorbance at 670 nm

Sample Type : Urease inhibitors

Species Reactivity : Mammalian

Applications : Screening or characterizing urease inhibitors.

Features and Benefits : A control Urease Inhibitor is also included in the kit. A quick, easy and sensitive assay to screen and characterize potential urease inhibitors.