USP25 Antibody | 7765

USP25 Antibody | 7765 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.
The immunogen is located within amino acids 470 - 520 of USP25.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 116 kDa
Observed: 105 kDa

Validation: N/A

Isoform: N/A

Purification: USP25 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: USP25 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: USP25 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: USP25 Antibody: USP21, USP21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: USP25 (ubiquitin specific peptidase 25) , also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1) . It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2) . The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3, 4) .