V-ATPase | Epsilon subunit of tonoplast H+ATPase (goat antibody) | AS09 577

V-ATPase | Epsilon subunit of tonoplast H+ATPase (goat antibody) | AS09 577 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1) .

Host: Goat

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS07 213 | V-ATPase | epsilon subunit of tonoplast H+ATPase rabbit antibodiesAS08 577A | V-ATPase | epsilon subunit of tonoplast H+ATPase goat antibodies, affinity purifiedcollection of antibodies to membrane transport systemmarker antibodies for plant cellular compartmentsPlant protein extraction buffer

Recommended Dilutions: 1 : 1000-1 : 3000 (WB)

Molecular weight: 26 | 31 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum

Predicted Reactivity: Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp., Phaseolus sp., Physcomitrium patens, Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Reconstitution: For reconstitution add 300 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G11150

Category: Vacuolar membrane

Research Area: Plant Biology