VASP Antibody [IE273] | 36-170

VASP Antibody [IE273] | 36-170 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Bovine, Human, Pig, Rabbit, Sheep

Homology: N/A

Immunogen: Purified human VASP.

Research Area: Cancer, Immunology, Innate Immunity

Tested Application: E, ICC, IHC, IP, WB

Application: Immunocytochemistry: (1-5ug/ml, 1:100-1:500) . Western Blot: (0.01-0.25ug/ml, 1:2000-1:50, 000) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of human, porcine, bovine, sheep and rabbit VASP.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein A/G purified.

Clonality: Monoclonal

Clone: IE273

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 2mg/ml BSA and 0.02% sodium azide.

Concentration: 500 ug/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: Vasodilator-stimulated Phosphoprotein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.