VEGF121 protein (His tag) | 80R-1082

VEGF121 protein (His tag) | 80R-1082 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Conjugated Proteins

Research Area: Proteases, Inhibitors, & Enzymes

Short Description: Purified recombinant Human VEGF121 protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 207-327 amino acids: MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

Tag/conjugate: His tag

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 90% pure

Methode of Purification: N/A

Source: N/A

Concentration: 0.5 mg/ml

Form & Buffer: Liquid in 20mM Tris pH 8.0  containing 10% glycerol

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.

Usage Recommendations: N/A

Assay Information: Cell line: NIH-3T3 (Mus mosculus/ embryo) Maintenance Condition: RPMI 1640 containing 10% FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10, 000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation Time: 24 hr (after sample treatment) Concentration Range: 100 pg/ml - 10 ug/ml Detection method: BrdU assay