VIM Polyclonal Antibody | E-AB-18212

VIM Polyclonal Antibody | E-AB-18212 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME, Vimentin

Application: WB, IHC, ELISA

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Reserch Areas: Cancer, Developmental Biology, Neuroscience, Signal Transduction, Stem Cells

Background: Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Fusion protein of human VIM

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification Method: Antigen affinity purification

Dilution: WB 1:2000-1:5000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 54 kDa

Molecular Weight(Observed): Refer to figures