Vimentin, Human Recombinant | P1012

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

P1012 | Vimentin Human Recombinant DataSheet

Biomolecule/Target: Vimentin

Synonyms: VIM

Alternates names: Thymus Expressed Chemokine, CCL25

Taglines: A class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

NCBI Gene ID #: 6370

NCBI Gene Symbol: CCL25

Gene Source: Human

Accession #: O15444

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 14.2 kDa

Concentration: N/A

Appearance: Dry powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic acid.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL