Visfatin, Rat Recombinant | 4909

Nicotinamide phosphoribosyltransferase (NAMPT), also known as visfatin or pre-B-cell colony enhancing factor, is a protein that catalyzes the formation of nicotinamide mononucleotide as part of the biosynthetic pathway that produces nicotinamide adenine dinucleotide (NAD). This protein is thought to be involved in many important biological processes, including metabolism, stress response, and aging. The secreted form of the protein acts as a cytokine to promote B cell maturation and inhibits neutrophil apoptosis. Visfatin operates as a circadian clock modulator through NAD-mediated oscillation of clock target gene expression such as CLOCK and ARNTL through a SIRT1-mediated mechanism.

4909 | Visfatin rat recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1 homolog, NAmPRTase, Nampt, PBEF

Alternates names: T-4, Hematopoietic system regulatory peptide, Seraspenide, TMSB4X, TB4X, TMSB4

Taglines: A cytokine that promotes B cell maturation and inhibits neutrophil apoptosis

NCBI Gene ID #: 7114

NCBI Gene Symbol: TMSB4X

Gene Source: Human

Accession #: P62328

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Pretreatment of primary lung fibroblasts with recombinant Thymosin 4, using a concentration of 0.5 - 10 ug/ml, produces a protective effect against hydrogen peroxide induced cell death.

Biological activity: Pretreatment of primary lung fibroblasts with recombinant Thymosin 4, using a concentration of 0.5 - 10 ug/ml, produces a protective effect against hydrogen peroxide induced cell death.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 5.2 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: RMSDKPDMAE IEKFDKSKLK KTETQEKNPL PSKETIEQEK QAGES