VPS4A Antibody (N-term) [APG03019G]

VPS4A Antibody (N-term) [APG03019G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This VPS4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-77amino acids from the N-terminal region of human VPS4A.

Cellular Localisation: Late endosome membrane {ECO:0000250|UniProtKB:Q8VEJ9}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8VEJ9}. Midbody Note=Membrane-associated in the prevacuolar endosomal compartment Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515) Localized in two distinct rings on either side of the Flemming body

Molecular Weight: 48898

Clone: Polyclonal

Gene Name: VPS4A {ECO:0000312|EMBL:AAG01470.1}

Gene ID: 27183

Function: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515) . VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413) .

Summary: Tissue Location: Ubiquitously expressed.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Vacuolar protein sorting-associated protein 4A, Protein SKD2, VPS4-1, hVPS4, VPS4A {ECO:0000312|EMBL:AAG014701}