VTG | Sole vitellogenin | AS06 127

VTG | Sole vitellogenin | AS06 127 | Gentaur UK, US & Europe Distribution

Immunogen: native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to fish proteinsSecondary antibodies

Recommended Dilutions: 1 : 5 000 on sole serum (ELISA), 1 : 5 000 (WB)

Molecular weight: ca, 200 kDa

Confirmed Reactivity: Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: The developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

Background: Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen) . However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Fish Proteins

Research Area: Cell Biology