Description
WAC Antibody | 7293 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: WAC antibody was raised against a 19 amino acid peptide near the center of human WAC .
The immunogen is located within amino acids 210 - 260 of WAC.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB, IHC-P, IF
Application: WAC Antibody can be used for detection of WAC by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of WAC are known to exist; this antibody will detect the larger isoform.
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 71 kDa
Observed: 75 kDa
Validation: N/A
Isoform: N/A
Purification: WAC Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: WAC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: WAC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: WAC Antibody: Wwp4, BM-016, PRO1741, KIAA1844, WW domain-containing adapter protein with coiled-coil
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: WAC Antibody: The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.