WAC Antibody (Center) [APR32711G]

WAC Antibody (Center) [APR32711G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This WAC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-313 amino acids from the Central region of human WAC.

Cellular Localisation: Nucleus speckle {ECO:0000250|UniProtKB:Q924H7}. Nucleus. Note=In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes {ECO:0000250|UniProtKB:Q924H7}

Molecular Weight: 70724

Clone: Polyclonal

Gene Name: WAC

Gene ID: 51322

Function: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1) (PubMed:21329877) . Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription (PubMed:21329877) . Regulates the cell-cycle checkpoint activation in response to DNA damage (PubMed:21329877) . Positive regulator of amino acid starvation-induced autophagy (PubMed:22354037) . Also acts as a negative regulator of basal autophagy (PubMed:26812014) . Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation (PubMed:26812014) . May negatively regulate the ubiquitin proteasome pathway (PubMed:21329877) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: WB--1:1000 FC--1:10~50

Synonyms: WW domain-containing adapter protein with coiled-coil, WAC, KIAA1844