WDR40A Antibody (Center) [APR04080G]

WDR40A Antibody (Center) [APR04080G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This WDR40A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human WDR40A.

Cellular Localisation: Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Molecular Weight: 50517

Clone: Polyclonal

Gene Name: DCAF12 {ECO:0000303|PubMed:16949367, ECO:0000312|HGNC:HGNC:19911}

Gene ID: 25853

Function: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:16949367, PubMed:16964240, PubMed:29779948) . The C- degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948) . The DCX (DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:31267705) . Ubiquitination of MAGEA3, MAGEA6 by DCX (DCAF12) complex is required for starvation-induced autophagy (PubMed:31267705) .

Summary: Tissue Location: Highly expressed in lung cancer tissues and some cancer cell lines (PubMed:18957058) . Restricted expression in normal testis (PubMed:18957058) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: DDB1- and CUL4-associated factor 12, Centrosome-related protein TCC52, Testis cancer centrosome-related protein, WD repeat-containing protein 40A, DCAF12, KIAA1892, TCC52, WDR40A