WDR61 Antibody | 7985

WDR61 Antibody | 7985 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (93%) , Chicken: (79%)

Immunogen: WDR61 antibody was raised against a 14 amino acid peptide near the amino terminus of human WDR61.
The immunogen is located within the first 50 amino acids of WDR61.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: WDR61 antibody can be used for detection of WDR61 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: WDR61 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 34 kDa
Observed: 30 kDa

Validation: N/A

Isoform: N/A

Purification: WDR61 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: WDR61 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: WDR61 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: WD repeat-containing protein 61, Meiotic recombination REC14 protein homolog, REC14, SKI8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: WDR61 (WD-repeat-containing protein 61) , also known as SKI8 or REC14, is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (1, 2) . PAF1C is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (3, 4) .