WNK1 Antibody (C-Term) [AMM08518G]

WNK1 Antibody (C-Term) [AMM08518G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This WNK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1848-1882 amino acids from human WNK1.

Cellular Localisation: Cytoplasm.

Molecular Weight: 250794

Clone: Polyclonal

Gene Name: WNK1 (HGNC:14540)

Gene ID: 65125

Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity) .

Summary: Tissue Location: Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney- specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Serine/threonine-protein kinase WNK1, 2.7.11.1, Erythrocyte 65 kDa protein, p65, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, hWNK1, WNK1, HSN2, KDP, KIAA0344, PRKWNK1