Wnt-1 Antibody | 5754

Wnt-1 is a secreted protein that signals thro?gh the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.

5754 | Wnt-1 Antibody DataSheet

Antibody Target: Wnt-1

Target Alternative Name: wnt1, wnt 1, wnt-1, INT 1 , INT1, wingless type MMTV integration site family member 1

Tag Line:

Category: Wnt

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: E. coli expressed human Wnt-1

Accession #: P04628

Gene ID: 7471

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-human Wnt-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control:

Application And Usages: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human Wnt-1 (Cat.# 4754-10) can be used as a positive control.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified