WNT-7a, Human Recombinant | P1195

WNT7A is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. Recombinant human WNT7A protein, fused to His-tag at N-terminus, was expressed in E.coli.

P1195 | WNT-7a Human Recombinant DataSheet

Biomolecule/Target: WNT7A

Synonyms: WNT7A, Protein Wnt-7a

Alternates names: TNFSF15, VEGI

Taglines: Plays a key role in oncogenesis and embryogenesis

NCBI Gene ID #: 9966

NCBI Gene Symbol: TNFSF15

Gene Source: Human

Accession #: O95150

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate IFN- production by human PBMC using a concentration range of 10.0-100.0 ng/ml. Note: Results may vary with PBMC donors.

Biological activity: Determined by its ability to stimulate IFN- production by human PBMC using a concentration range of 10.0-100.0 ng/ml. Note: Results may vary with PBMC donors.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 22.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 8.5, and 75 mM Arginine.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL