WNT5 Antibody | 8063

WNT5 Antibody | 8063 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (93%)

Immunogen: WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B.
The immunogen is located within amino acids 130 - 180 of WNT5.

Research Area: Cancer, Cell Cycle, Stem Cell

Tested Application: E, WB, IHC-P

Application: WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Specificiy: WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 39 kDa
Observed: 465 kDa

Validation: N/A

Isoform: N/A

Purification: WNT5 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: WNT5 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: WNT5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Wingless-type MMTV integration site family member 5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1, 2) . WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3) . Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4) .