Description
WRNIP1 Antibody | 46-600 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: EELRGVDFFKQRRC
Research Area: Cancer
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1kDa according to NP_064520.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol Chem. 2007 Aug 10;282 (32) :23184-93; PMID: 17550899) . Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6ug/ml.
Specificiy: This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: WHIP, Werner helicase interacting protein, FLJ22526, putative helicase RUVBL, Werner helicase interacting protein 1, RP11-420G6.2, bA420G6.2,
User Note: N/A
BACKGROUND: N/A