WSB1 Antibody | 8161

WSB1 Antibody | 8161 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (82%)

Immunogen: WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1.
The immunogen is located within amino acids 70 - 120 of WSB1.

Research Area: Homeostasis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: WSB1 antibody is human specific.

Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 46 kDa

Validation: N/A

Isoform: N/A

Purification: WSB1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: WSB1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: WSB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: WD repeat and SOCS box containing 1, SOCS box-containing WD protein SWiP-1, SWIP1, WD repeat and SOCS box-containing protein 1, WSB-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1) , is a member of the WD-protein subfamily (1) . It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1, 2) . WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3) . Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4, 5) .