WST-1 | 2198

A water-soluble tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 438 nM. Useful in cell proliferation assays as a cell viability indicator.

WST-1 is a water-soluble tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. Useful in cell proliferation assays as a cell viability indicator.

2198 | WST-1 DataSheet

Alternate Name/Synonyms: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt

Appearance: Pale brown solid

Formulation: N/A

CAS Number: 150849-52-8

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₉H₁₁IN₅NaO₈S₂

Molecular Weight: 651.35

Cell-Permeable?: Yes

Purity: ≥97% by HPLC

Solubilities: H2O (10 mg/ml); 6.5 mg/ 10ml 50mM Tris buffer, pH 8.0

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A sensitive chromogenic indicator for NADH

MDL Number: MFCD01075079

PubChem CID: 6099081

SMILES: C1=CC(=CC=C1N2N=C(N=[N+]2C3=CC=C(C=C3)I)C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-])[N+](=O)[O-].[Na+]

InChi: InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33;/h1-11H,(H-,28,29,30,31,32,33);/q;+1/p-1

InChi Key: JUJBNYBVVQSIOU-UHFFFAOYSA-M