WST-8 | 2049

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful in cell proliferation assays as a cell viability indicator.

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful in cell proliferation assays as a cell viability indicator.

2049 | WST-8 DataSheet

Alternate Name/Synonyms: 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt

Appearance: Light pink solid

Formulation:

CAS Number: 193149-74-5

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₀H₁₃N₆O₁₁S₂ • Na

Molecular Weight: 600.47

Cell-Permeable?: TRUE

Purity: ≥98% by HPLC

Solubilities: ~50 mg/ml Water, ~10 mg/ml DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A sensitive chromogenic indicator for NADH

MDL Number:

PubChem CID: 9894947

SMILES: [O-]S(C1=CC(S([O-])(=O)=O)=C(C2=NN(C3=CC=C([N+]([O-])=O)C=C3)[N+](C4=C(OC)C=C([N+]([O-])=O)C=C4)=N2)C=C1)(=O)=O.[Na+]

InChi: InChI=1S/C20H14N6O11S2.Na/c1-37-18-10-14(26(29)30)6-9-17(18)24-22-20(21-23(24)12-2-4-13(5-3-12)25(27)28)16-8-7-15(38(31,32)33)11-19(16)39(34,35)36;/h2-11H,1H3,(H-,31,32,33,34,35,36);/q;+1/p-1

InChi Key: VSIVTUIKYVGDCX-UHFFFAOYSA-M