WT1 Antibody (Center E361) [APR03642G]

WT1 Antibody (Center E361) [APR03642G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.

Cellular Localisation: Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190) . Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269|PubMed:15520190} [Isoform 4]: Nucleus, nucleoplasm

Molecular Weight: 49188

Clone: Polyclonal

Gene Name: WT1

Gene ID: 7490

Function: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533) . Recognizes and binds to the DNA sequence 5'-GCG (T/G) GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363) . Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190) . Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801) . Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921) .

Summary: Tissue Location: Expressed in the kidney and a subset of hematopoietic cells

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, IF, FC

Dilution: WB--1:1000 FC--1:10~50 IHC-P--1:100~500 IF--1:10~50

Synonyms: Wilms tumor protein, WT33, WT1