WT1 Antibody (Wilms Tumor protein)| F41819-0.08ML/F41819-0.4ML

WT1 Antibody (Wilms Tumor protein)| F41819-0.08ML/F41819-0.4ML | Gentaur UK, US & Europe Distribution

Background: Wilms Tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: Mouse, Rat, Pig, Xenopus

Application: IF, FACS, WB, IHC, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

Application Note: Titration of the WT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This WT1 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 346-375 from the human protein was used as the immunogen for this WT1 antibody.

Storage: Aliquot the WT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A