WWC1 Antibody | 7991

WWC1 Antibody | 7991 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1.
The immunogen is located within amino acids 60 - 110 of WWC1.

Research Area: Homeostasis, Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 123 kDa
Observed: 110 kDa

Validation: N/A

Isoform: N/A

Purification: WWC1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: WWC1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: WWC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: WW and C2 domain containing 1, HBEBP3, HBEBP36, KIBRA, MEMRYQTL

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1) . WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3) . WWC1 is phosphorylated by the mitotic kinases Aurora-A and –B (4) , and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5) .