WWTR1 Antibody (C-term) [AMM08526G]

WWTR1 Antibody (C-term) [AMM08526G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This WWTR1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 296-330 amino acids from the C-terminal region of human WWTR1.

Cellular Localisation: Nucleus. Cytoplasm. Cell membrane. Note=Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies (By similarity) . When phosphorylated, is retained in the cytoplasm by YWHAZ (By similarity) . Can be retained in the nucleus by MED15 (PubMed:18568018) . Localized in the cytoplasm in areas of epithelial cell high density (PubMed:21145499) . At blastocyst stage expressed in the nucleus in trophectodermal cells, however expressed in the cytoplasm in the inner cell mass (By similarity) {ECO:0000250|UniProtKB:Q9EPK5, ECO:0000269|PubMed:18568018, ECO:0000269|PubMed:21145499}

Molecular Weight: 44101

Clone: Polyclonal

Gene Name: WWTR1

Gene ID: 25937

Function: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151) . The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151) . WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321) . In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018) . Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018) . Regulates embryonic stem-cell self- renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018) .

Summary: Tissue Location: Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, WWTR1, TAZ