Xylose | AS07 267

Xylose | AS07 267 | Gentaur UK, US & Europe Distribution

Immunogen: xylose residues bound to the N-glycan in beta 1, 2

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS07 268 | Anti-fucose, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 µg/ml (ELISA), 2 µg/10 ml incubation buffer (WB)

Molecular weight: 10-100 for various glycoproteins

Confirmed Reactivity: Higher plants and algae

Predicted Reactivity: Higher plants

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.Positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

Background: This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1, 2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Carbohydrates

Research Area: Plant Biology