YB1 (YBX1) Antibody (N-term) [APR05739G]

YB1 (YBX1) Antibody (N-term) [APR05739G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This YB1 (YBX1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human YB1 (YBX1) .

Cellular Localisation: Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome Note=Predominantly cytoplasmic in proliferating cells (PubMed:12604611) . Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551) . Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427) . Shuttles between nucleus and cytoplasm (PubMed:25229427) . Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541) . Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673)

Molecular Weight: 35924

Clone: Polyclonal

Gene Name: YBX1 (HGNC:8014)

Gene ID: 4904

Function: DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:8188694, PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969) . Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969) . Promotes mRNA stabilization: acts by binding to m5C- containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969) . Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303) . Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity) . Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095) . Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C) -containing RNAs (PubMed:28341602, PubMed:29073095) . Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925) . Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611) . Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed:18809583) . Binds to promoters that contain a Y-box (5'- CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:8188694, PubMed:18809583) . Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551) . Has endonucleolytic activity and can introduce nicks or breaks into double- stranded DNA, suggesting a role in DNA repair (PubMed:14718551) . The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1