ZFX Antibody | 6811

ZFX Antibody | 6811 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: ZFX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFX.
The immunogen is located within the last 50 amino acids of ZFX.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: ZFX antibody can be used for detection of ZFX by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ZFX antibody is human, mouse and rat reactive. Multiple isoforms of ZFX are known to exist. ZFX antibody will detect isoform 1 and 2 of ZFX.

Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 2: Cat. No. 1223 - Caco-2 Cell Lysate

Positive Control 3: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 89, 63 kDa
Observed: 63 kDa

Validation: N/A

Isoform: N/A

Purification: ZFX Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ZFX Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ZFX antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ZFX Antibody: ZNF926, Zinc finger X-chromosomal protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ZFX Antibody: The X-linked zinc finger protein ZFX is a member of the Kruppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD) , a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny.