Description

Zink assay kit (5-Br-PAPS)

[ Product Description ]

Zinc is an essential mineral cofactor of more than 200 enzymes which involved in nucleic acid, and protein synthesis. In mammals, acute zinc deficiency during the growth period is reported to cause failure in skin and hair, and growth retardation. This kit is a simple colorimetric assay which can detect zinc precisely in biological fluids in a short period of time (about 10 minutes).

[ Assay principle ]

Protein-bonded zinc is dissociated by denaturing agent. Free and protein-bonded zinc are mixed with 5-Br-PAPS to form chelate complex. Zinc-5-Br-PAPS complex can be detected by measuring absorbance at 560 nm.

[ Performance ]

1. Sensitivity: 2. Accuracy: 3. Intra assay variation: 4. Assay range:	O.D. at 200 µg/dL standard will be 0.1 - 0.3 ±15% of known concentration C.V. is less than 5% 4 - 1,000 µg/dL
This assay will not be affected by following substances. 1. Hemoglobin: up to 0.5 g/dL 2. Bilirubin: up to 15 mg/dL 3. Triglyceride: up to 1000 mg/dL This assay will be interfered by EDTA.

[ Specifications ]

Assay principle:	5-Br-PAPS method (Colorimetric assay at 560 (550-580) nm)
Specifity:	Zinc (Zn ²⁺)
Measuring range:	4 - 1,000 µg/dL
Time to answer:	10 minutes at room temperature (25 - 37 °C)
Applications:	Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Will be interfered by EDTA.
Storage temperature:	2 - 8°C (don't freeze).
Expiry date:	1 year after the day of manufacturing.
Required but not provided:	Distilled water 96 well microtiter plate Micropipettors and pipette tips Microtiter plate reader (measuring wavelength 560 nm)

[ Contents of this kit ]

R-1 Buffer (ready to use):	24 mL
R-2 Chelate color (ready to use):	0.5 mL
Zinc standard 200 µg/dL (ready to use):	1.2 mL

[ References: Assay examples ]

1)	Kazuma Nakanishi, Masahiro Tomita, Katsuya Kato: Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture. Journal of Asian Ceramic Societies 3(1), p70-76(2015) doi:10.1016/j.jascer.2014.10.011
2)	Akira Mochizuki and Hideki Kaneda: Study on the blood compatibility and biodegradation properties of magnesium alloys. Materials Science and Engineering: C 47(1), p204-210(2015) doi:10.1016/j.msec.2014.11.041
3)	Yasuyuki Sakurai, Itsuki Anzai and Yoshiaki Furukawa: A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase. J. Biol. Chem. published online June 10, 2014 doi: 10.1074/jbc.M114.567677
4)	Akira Minami, Hiroshi Matsushita, Yuuki Horii, Daisuke Ieno, Yukino Matsuda, Masakazu Saito, Hiroaki Kanazawa, Yuriko Ohyama, Akihiko Wakatsuki, Atsushi Takeda, Kazuya I. P. J. Hidari, Vikineswary Sabaratnam, Takashi Suzuki: Improvement of Depression-Like Behavior and Memory Impairment with the Ethanol Extract of Pleurotus eryngii in Ovariectomized Rats. Biological and Pharmaceutical Bulletin 36(12),p1990-1995(2013) doi: 10.1248/bpb.b13-00657
5)	Homma K, Fujisawa T, Tsuburaya N, Yamaguchi N, Kadowaki H, Takeda K, Nishitoh H, Matsuzawa A, Naguro I, Ichijo H: SOD1 as a molecular switch for initiating the homeostatic ER stress response under zinc deficiency. Mol Cell 52(1),p75-86(2013) doi: 10.1016/j.molcel.2013.08.038
6)	Noriyuki Isobe, Xiaoxia Chen, Ung-Jin Kim, Satoshi Kimura, Masahisa Wada, Tsuguyuki Saito, Akira Isogai: TEMPO-oxidized cellulose hydrogel as a high-capacity and reusable heavy metal ion adsorbent. Journal of Hazardous Materials 260, p195-201(2013) doi:10.1016/j.jhazmat.2013.05.024
7)	Joshua Chou, Jia Hao, Hirokazu Hatoyama, Besim Ben-Nissan, Bruce Milthorpe, Makoto Otsuka: The Therapeutic Effect on Bone Mineral Formation from Biomimetic Zinc Containing Tricalcium Phosphate (ZnTCP) in Zinc-Deficient Osteoporotic Mice. PLoS ONE 8(8): e71821. doi:10.1371/journal.pone.0071821
8)	Fei Zhuang and Nobutaka Hanagata: Synergic Toxicity of Solid Particles and Released Zinc from Zinc Oxide Nanoparticles to Human Lung Epithelial Cells. Nano Biomedicine 4(2)p90-112(2012) doi: 10.11344/nano.4.90

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Additional Information

Size:	100 Tests
Type of Marker:	Trace elements assay
Marker:	Ca
Storage:	2 - 8°C (don't freeze).
Colorimetric:	680-700 nm
Assay range:	0.05-20 mg/dL
Usage:	Urine, Serum, Tissue
Remark:	Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples.