Description
ZNRF1 Antibody | 43-275 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-SDSTYAHGNGYQET
Research Area: Obesity, Signal Transduction
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel) , consistent with the observed band in transfected N2a cells transiently expressing Znrf1 (left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, JapanImmunohistochemistry:In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human Brain (Cerebellum) . Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1) . Reported variants represent identical protein: NP_001162092.1, NP_573469.1
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Approx 28kDa
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: Znrf1, zinc and ring finger 1, B830022L21Rik, MGC101991, Rnf42, Zrfp1, nin283, E3 ubiquitin-protein ligase ZNRF1, nerve injury-induced gene 283 protein, peripheral nerve injury protein nin283, ring finger protein 42, zinc ring finger protein 1, zinc/RING finger protein 1
User Note: N/A
BACKGROUND: N/A