Description
ZO-1 Antibody | 42-230 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-PKPTSQNQFSEHDKT
Research Area: Membrane
Tested Application: E, IF, WB
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2) . Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cell junctions of A431 cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)
Positive Control 1: Cat. No. XBL-10106 - Human Cerebral Cortex Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: TJP1, tight junction protein 1 (zona occludens 1) , DKFZp686M05161, MGC133289, ZO-1, OTTHUMP00000174520, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
User Note: N/A
BACKGROUND: N/A