A431 Lysate | 1202

A431 Lysate | 1202 | Gentaur UK, US & Europe Distribution

Tested Application: WB

Application: N/A

Storage Condition: Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

User Note: Note: Lysate lane order may vary; please consult accompanying shipment paperwork.

Background: The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are Daudi (B lymphoblast; Burkkit's lymphoma) , Raji (B lymphoblast; Burkkit's lymphoma) , K-562 (bone marrow; chronic myelogenous leukmia) , U-937 (histiocytic lymphoma) , THP-1 (monocyte; acute monocytic leukmia) , HL-60 (promyeloblast; acute promyeloblastic leukmia) , Jurkat (T lymphocyte; acute T cell leukmia) , and MOLT-4 (T lymphoblast; acute lymphoblastic leukmia) .

Shipping: N/A

Type: Cell Line

Physical State: Liquid

Buffer: A431 lysate is supplied in SDS sample buffer containing 50 mM DTT.

Concentration: 2 mg/mL

Background: A431 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The A431 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Disclaimer: This product is intended for laboratory research purposes only and should be used by qualified personnel only. ProSci lysates are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci lysates.

Short Description: epidermoid carcinoma

Source: N/A

Tissus By Diagnosis: Diseased

Tissus By Developemental Stage : N/A

Tissus By Species: Human

Tissus/ Cell: A431 (Human Epidermoid Carcinoma)

Diagnostic: Tumor

Cancer Type: N/A

Cancer Type Ezpanded: N/A

Age: N/A

Sex: N/A

Race: N/A

Species: Human