Active Human Calpain 1 | 1134

Ca²-dependent cysteine proteinase with low Ca²requirement (half maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin. Involves in apoptosis activation.

1134 | Active Human Calpain 1 DataSheet

Biomolecule/Target: Calpain 1

Synonyms: Calpain

Alternates names: CANP; muCL; CANP1; SPG76; CANPL1; muCANP; Calpain mu-type; calcium-activated neutral proteinase 1; calpain-1 catalytic subunit; calpain-1 large subunit; cell proliferation-inducing gene 30 protein; micromolar-calpain

Taglines: A cysteine protease involved in apoptosis activation

NCBI Gene ID #: 6375

NCBI Gene Symbol: XCL1

Gene Source: Human

Accession #: P47992

Recombinant: No

Source: Human Erythrocyte

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): >1000 units/mg protein

Biological activity: Determined by its ability to chemoattract human peripheral blood lymphocytes using a concentration range of 10-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze one pmol Suc-LLVY-AMC per minute at 25 °C.

Molecular Weight: 10.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM imidazole-HCl, 5 mM -mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A