Active Porcine Calpain 1 | 1138

The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.

1138 | Active Porcine Calpain 1 DataSheet

Biomolecule/Target: Calpain 1

Synonyms: Calpain 1

Alternates names: Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain

Taglines: A cysteine protease involved in apoptosis activation

NCBI Gene ID #: 397027

NCBI Gene Symbol: CAPN1

Gene Source: Porcine

Accession #: P35750

Recombinant: No

Source: Porcine Erythrocytes

Purity by SDS-PAGEs: 85%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: 1000 units/mg protein prior to freezing.

Results: 1000 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25°C using the Calpain Activity Assay Kit, (K240-100).

Molecular Weight: 110 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM imidazole-HCl, 5 mM -mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.

Reconstitution Instructions: N/A

Amino acid sequence: N/A