Active PAD4, Human Recombinant | P1720

Protein arginine deiminases (PADs) are a family of guanidine-modifying enzymes that catalyze the post-translational modification of target proteins by converting arginine to citrulline. Excessive citrullination of proteins may result in disease states. PAD4 (also known as PADI5) is expressed in neutrophils and in a variety of tissues. PAD4 is known to citrullinate histones, which initiates chromatin decondensation in neutrophils and the subsequent release of neutrophil extracellular traps (NETs), which leads to a lytic form of cell death known as NETosis. The presence of serum PAD4 autoantibodies has been associated with rheumatoid arthritis.

P1720 | Active PAD4, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Peptidyl Arginine Deiminase 4; PDI5; Peptidyl Arginine Deiminase, Type IV; Peptidyl Arginine Deiminase, Type V; Protein-Arginine Deiminase Type IV; Protein-Arginine Deiminase Type-4; EC 3.5.3.15; HL-60 PAD; PADI5; PDI4; PAD; Peptidylarginine Deiminase IV; PADI-H Protein; PADI4

Alternates names:

Taglines: Protein arginine deiminase associated with rheumatoid arthritis

NCBI Gene ID #: 23569

NCBI Gene Symbol:

Gene Source: Human

Accession #: Q9UM07

Recombinant: True

Source: E.coli

Purity by SDS-PAGE: ≥ 85% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): Activity output measured by the production of ammonium ions when enzyme acts on N-benzoyl-L-arginine ethyl ester (BAEE) substrate

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 75.8 kDa

Concentration:

Appearance: Liquid protein

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: Full length human PAD4 protein (amino acids 2 to 663) with N-terminal His tag

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans