Active SIRT6 (GST-tagged), human recombinant | 7697

Human Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards Lys-9 and Lys-56 of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-.

7697 | Active SIRT6 GST-tagged human recombinant DataSheet

Biomolecule/Target: SIRT6

Synonyms: SIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6

Alternates names: SIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6

Taglines: GST tagged Human SIRT6 from E.Coli, AOF, 85% pure, biologically active.

NCBI Gene ID #: 50498

NCBI Gene Symbol: EBI3

Gene Source: Human

Accession #: Q3U1K3

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): ’s Active GST-tagged SIRT6 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >3 mU/mg (One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C).

Biological activity: Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Results: >3 mU/mg

Binding Capacity: N/A

Unit Definition: One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C

Molecular Weight: 22.9 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: Human SIRT6 protein is a solution of 1 mg/ml in 50 mM Tris, 100 mM NaCl, pH 8.0 containing 20% glycerol.

Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 – 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A