Active HIV1 Protease Recombinant (GST-tagged) | 7849

HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV. HIV protease cleaves newly synthesized viral polyproteins at the appropriate places into functional protein products as mature protein components of an infectious HIV virion. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells.

7849 | Active HIV1 Protease Recombinant GST-tagged DataSheet

Biomolecule/Target: HIV1 Protease

Synonyms: HIV-1 retropepsin, HIV-1 Protease (PR), cd05482

Alternates names: HIV-1 retropepsin, HIV-1 Protease (PR), cd05482

Taglines: 90% Pure Active Recombinant HIV1 Protease (GST tagged) for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.

NCBI Gene ID #: 3604

NCBI Gene Symbol: TNR9

Gene Source: HIV1

Accession #: Q07011

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.8 kDa

Concentration: 3 mg/ml

Appearance: Liquid

Physical form description: 3 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A