EZCut™ SUMO Protease 1 (GST-tagged), Yeast Recombinant | 7869

SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. ’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal GST-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Glutathione resin (Cat. # 6555-1,10,50).

7869 | EZCut SUMO Protease 1 GST-tagged Yeast Recombinant DataSheet

Biomolecule/Target: SUMO Protease 1

Synonyms: Ulp1 peptidase, SUMO Protease

Alternates names: GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy,

Taglines: >90% Pure, Active, Yeast Recombinant, GST-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.

NCBI Gene ID #: 2660

NCBI Gene Symbol: MSTN

Gene Source: Yeast

Accession #: O14793

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The EZCut™ SUMO Protease 1 (GST-tagged) has an activity of 10,000 units/mg.

Biological activity: Test in process

Results: 10,000 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.

Molecular Weight: 27.8 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A