Active Sonic Hedgehog (SHH), human recombinant | 4010

Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.

4010 | Active Sonic Hedgehog SHH human recombinant DataSheet

Biomolecule/Target: Sonic Hedgehog (SHH)

Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog

Alternates names: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog

Taglines: Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis

NCBI Gene ID #: 5473

NCBI Gene Symbol: NAP2

Gene Source: Human

Accession #: P02775

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: <0.01 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0-10 ng/ml.

Results: 750ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.6 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20° for future use.

Amino acid sequence: N/A