Active Sonic Hedgehog (SHH), murine recombinant | 4020

Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.

4020 | Active Sonic Hedgehog SHH murine recombinant DataSheet

Biomolecule/Target: Sonic Hedgehog (SHH)

Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog

Alternates names: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog

Taglines: Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis

NCBI Gene ID #: 20423

NCBI Gene Symbol: SHH

Gene Source: Mouse

Accession #: Q8C765

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: <0.01 ng/g

Activity (Specifications/test method): N/A

Biological activity: Mouse SHH is fully biologically active when compared to standard. Determined by it’s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED in the range of 0.03 – 0.30 ug/ml.

Results: 0.03 – 0.30 ug/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.

Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile HO to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A