Activin A, human recombinant | 4724

Activin A is a member of the TGFbeta family of proteins produced by many cell types throughout development. Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc. Activin A is a homodimer of two beta A chains and is not biologically active until the N terminal propeptide is cleaved from each. Human Activin A has 100% amino acid sequence identity to mouse, rat, porcine, bovine and feline proteins. Recombinant human Activin A is a non-glycosylated homodimer, containing two 117 amino acid chains, with a total molecular weight of 26.2 kDa.

4724 | Activin A human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Inhibin beta-1, FRP, FSH-releasing protein

Alternates names: Inhibin beta-1, FRP, FSH-releasing protein

Taglines: A protein complex enhancing FSH biosynthesis and secretion

NCBI Gene ID #: 6387

NCBI Gene Symbol: SDF1A

Gene Source: Human

Accession #: P48061

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: <0.1 EU/g purified protein

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 20-80 ng/ml.

Results: < 2 ng/mL

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Recombinant human Activin A is lyophilized with 0.02% TFA.

Reconstitution Instructions: Reconstitution in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A