Activin B, Human Recombinant | 7112

Activin B is a TGF- family member that exhibits a wide range of biological activities including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF- family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 25.6 kDa disulfide-linked homodimer consisting of two B chains, each containing 115 amino acid residues.

7112 | Activin B Human Recombinant DataSheet

Biomolecule/Target: Activin B

Synonyms: Activin beta-B, Inhibin beta-2

Alternates names: Activin beta-B, Inhibin beta-2

Taglines: A TGF- family member involved in regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands.

NCBI Gene ID #: 20315

NCBI Gene Symbol: SDF1A

Gene Source: Human

Accession #: P40224

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is 2.0 ng/ml, corresponding to a specific activity of 5 x 105 units/mg.

Biological activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.9 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 5% Trehalose.

Reconstitution Instructions: Reconstitution in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers

Amino acid sequence: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK