AGO1 | Argonaute 1 (40 µg, ALP-conjugated) | AS09 527-ALP

AGO1 | Argonaute 1 (40 µg, ALP-conjugated) | AS09 527-ALP | Gentaur UK, US & Europe Distribution

Immunogen: KLH conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 UniProt:O04379, TAIR: At1g48410.

Host: Rabbit

Conjugation: ALP

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.

Format: Liquid

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 116, 4 | 130 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii

Additional Information: AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) .This antibody is directly conjugated to Alkaline Phosphatase (ALP) .

Reconstitution: N/A

Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

TAIR Nnumbre: AT1G48410

Category: microRNA

Research Area: RNAi/siRNA/miRNA