AGO1 | Argonaute 1 | AS09 527

AGO1 | Argonaute 1 | AS09 527 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Chromatin ImmunoPrecipitation (ChIP), Immunofluorescence (IF), Immunolocalization (IL), small-RNA-IP-Seq, Western blot (WB)

Related Products: AS09 527-ALP | Anti-AGO1 | Argonaute 1 (40 µg, ALP-conjugated), rabbit antibodiesAS09 527B | Anti-AGO1 | Argonaute 1 (40 µg, Biotin conjugated), rabbit antibodies AS09 527-HRP | Anti-AGO1 | Argonaute 1 (40 µg, HRP-conjugated), rabbit antibodiesAS14 2776 | Anti-AGO1 | Argonaute 1 (Chlamydomonas), rabbit antibodiesAS09 527P | AGO1 | Argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | Aargonaute 2, rabbit antibodoesAS09 617 | Anti-AGO4 | Argonaute 4, rabbit antibodiesAS10 671 | Anti-AGO5 | Argonaute 5, rabbit antibodiesAS10 672 | Anti-AGO6 | Argonaute 6, rabbit antibodiesAS10 673 | Anti-AGO9 | Argonaute 9, rabbit antibodiesAS15 3071 | Anti-AGO10 | Argonaute 10, rabbit antibodiesCollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 2 µg (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)

Molecular weight: 116, 4 | 130 kDa

Confirmed Reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana

Predicted Reactivity: Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays

Additional Information: AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) .

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G48410

Category: microRNA

Research Area: RNAi/siRNA/miRNA