AGO5 | Argonaute 5 | AS10 671

AGO5 | Argonaute 5 | AS10 671 | Gentaur UK, US & Europe Distribution

Immunogen: peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)

Related Products: AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodyAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction buffer

Recommended Dilutions: 1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)

Molecular weight: 111 | 111 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Arabidopsis thaliana

Not reactive in: Hordeum vulgare, Solanum lycopersicum, Zea mays

Additional Information: AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure

Background: AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) . Probably involved in antiviral RNA silencing.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G27880

Category: microRNA

Research Area: RNAi/siRNA/miRNA