AGO9 | Argonaute 9 | AS10 673

AGO9 | Argonaute 9 | AS10 673 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)

Related Products: AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodyAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction buffer

Recommended Dilutions: 5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)

Molecular weight: 101 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Arabidopsis thaliana

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Background: AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) .

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G21150

Category: microRNA

Research Area: RNAi/siRNA/miRNA