AIFM2 (CT) Antibody | 9653

AIFM2 (CT) Antibody | 9653 | Gentaur UK, US & Europe Distribution

Product Type: Primary Antibodies

Category: Primary Antibodies, Polyclonal Antibodies, Signal Transduction Antibodies

Research Area: Signal Transduction

Type: Polyclonal

Host Species: Rabbit

Reactivity Species: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Gorilla (100%) ; chimpanzee (100%), cow (92%), Horse (92%)

Immunology: Anti-AIFM2 antibody ( 9653) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2.

Tested Application: E, IHC, WB

Application Note: WB: 2 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human mouse and rat samples. All other applications and species not yet tested.

Positive Control 1: Cat. No. 1201 – HeLa Lysate

Positive Control 2: Cat. No. 17-001 – HeLa Cell Slide

Predicted Molecular Weight: Predicted: 41kD

Observed: 41 kD

Isoform: Human AIFM2 has 2 isoforms, including isoform 1 (373aa, 40.5kD) and isoform 2 (336aa, 36.5kD) . Mouse AIFM2 has 2 isoforms, isoform 1 (373aa, 40.6kD) and isoform 2 (380aa, 41.4kD) . Rat AIFM2 has one isoform (373aa, 40.7kD) . 9653 can detect AIFM2 in human, mouse and rat.

Purification: AIMF2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage: AIMF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Names: AIMF2

Protein Accession: Q9BRQ8

User Note: Optimal dilutions for each application to be determined by the researcher.

Background: AIFM2 Antibody: AIFM2 blocks ferroptosis independent of ubiquinol metabolism. AIFM2 is a key component of a non-mitochondrial CoQ antioxidant system that acts in parallel to the canonical glutathione-based GPX4 pathway. AIFM2 confers protection against ferroptosis elicited by GPX4 deletion. AIF-2 was found to be upregulated in human glioma tissues and cell lines. Downregulation of AIF-2 reduced cell proliferation and induced G1 cell cycle arrest. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type) . AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro) viral) cytosolic DNA, thus contributing to the onset of apoptosis

Loading Controls: N/A

Source: N/A

Purity: N/A

CAS: N/A

Shipping Condition: N/A