Description
AIFM2 (CT) Antibody | 9653 | Gentaur UK, US & Europe Distribution
Product Type: Primary Antibodies
Category: Primary Antibodies, Polyclonal Antibodies, Signal Transduction Antibodies
Research Area: Signal Transduction
Type: Polyclonal
Host Species: Rabbit
Reactivity Species: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Gorilla (100%) ; chimpanzee (100%), cow (92%), Horse (92%)
Immunology: Anti-AIFM2 antibody ( 9653) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2.
Tested Application: E, IHC, WB
Application Note: WB: 2 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human mouse and rat samples. All other applications and species not yet tested.
Positive Control 1: Cat. No. 1201 – HeLa Lysate
Positive Control 2: Cat. No. 17-001 – HeLa Cell Slide
Predicted Molecular Weight: Predicted: 41kD
Observed: 41 kD
Isoform: Human AIFM2 has 2 isoforms, including isoform 1 (373aa, 40.5kD) and isoform 2 (336aa, 36.5kD) . Mouse AIFM2 has 2 isoforms, isoform 1 (373aa, 40.6kD) and isoform 2 (380aa, 41.4kD) . Rat AIFM2 has one isoform (373aa, 40.7kD) . 9653 can detect AIFM2 in human, mouse and rat.
Purification: AIMF2 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage: AIMF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Names: AIMF2
Protein Accession: Q9BRQ8
User Note: Optimal dilutions for each application to be determined by the researcher.
Background: AIFM2 Antibody: AIFM2 blocks ferroptosis independent of ubiquinol metabolism. AIFM2 is a key component of a non-mitochondrial CoQ antioxidant system that acts in parallel to the canonical glutathione-based GPX4 pathway. AIFM2 confers protection against ferroptosis elicited by GPX4 deletion. AIF-2 was found to be upregulated in human glioma tissues and cell lines. Downregulation of AIF-2 reduced cell proliferation and induced G1 cell cycle arrest. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type) . AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro) viral) cytosolic DNA, thus contributing to the onset of apoptosis
Loading Controls: N/A
Source: N/A
Purity: N/A
CAS: N/A
Shipping Condition: N/A